Tm Calculator icon

This tool calculates the Tm of primers and estimates an appropriate annealing temperature when using different DNA polymerases. How to use this calculator

Quickly find the right annealing temperature for Platinum SuperFi DNA polymerase (also works for SuperScript IV One-Step RT-PCR Kit), Phusion and Phire DNA polymerases.

Important note: If the PCR primer contains desired mismatches, e.g., for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence

Note: Our standard Taq-based calculator is being upgraded and will be back online soon.

1. Select your DNA polymerase